Part:BBa_K5193003:Design

lip4 (Candida rugosa)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 815
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 841
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

We added a NSP4 tag (atgaaaaagattaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcg) before the sequence for better secretion of the protein.

Source

Source: Tang SJ, Sun KH, Sun GH, Chang TY, Lee GC. Recombinant expression of the Candida rugosa lip4 lipase in Escherichia coli. Protein Expr Purif. 2000 Nov;20(2):308-13. doi: 10.1006/prep.2000.1304. PMID: 11049754.